P C Ray Center for Research

ITM University, Gwalior, has established cutting-edge facilities and state-of-theart equipment to elevate standards in both teaching and research endeavors. The pinnacle of this initiative is the P.C. Ray Center for Research, ITM University, which houses an advanced analytical lab furnished with the latest instrumentation.

These facilities serve as a backbone for faculty members, research scholars, and students alike, fostering globally competitive research and development activities across a spectrum of basic and applied sciences. Notably, the equipment within the advanced analytical lab boasts exceptional features including fast scanning capabilities, high stability, and remarkable accuracy and reproducibility.

This infrastructure is not confined solely to the university community but extends its services to researchers from neighboring institutions, encouraging collaborative research initiatives. Recognizing the financial constraints often associated with procuring research instruments, ITM University is committed to spearheading outstanding research at the P.C. Ray Center for Research.

Faculty members and research scholars are actively involved in a diverse array of R&D activities spanning biotechnology, microbiology, biochemistry, plant science, animal science, environmental science, food technology, pharmaceutical science, physical science, and engineering & technology.

Various projects sanctioned by prestigious organizations such as MPCST, DST, AICTE, etc., are currently underway at ITM University. The availability of sophisticated equipment not only enhances research capabilities but also facilitates the successful execution of these projects.

These instruments find extensive applications across numerous sectors, including environmental science, chemical industries, agriculture, food industry, pharmaceuticals, material studies, quality control, clinical diagnosis, biochemistry, physical science, optical industries, and electronic engineering.

Moreover, the institution endeavors to extend its support to colleges, universities, and higher education centers by offering quality analysis of chemical compounds using these advanced instruments. Through such collaborative efforts, ITM University aims to cultivate a culture of innovation, excellence, and impactful research within the academic community.

PC Ray Research Center (PCRC) has been developed with the primary objective of fostering interdisciplinary research collaborations and driving innovation in various scientific disciplines.

• To strengthen technical infrastructure to carry out advanced research in various science disciplines.
• To carry out analysis of samples received from the academic institutes.
• To provide facilities of analytical instruments to academic institutes to enable them to carry outmeasurements for R&D work;
• To organize short-term training /workshops on the instrument handling and application of various instruments and analytical techniques;
• To train researchers for the maintenance and operation of instruments.

The research center is further open to work in collaboration with other university research institute industries to facilitate technology transfer from the lab field.

• Fourier-transform infrared spectroscopy (FTIR)
• UV-VIS spectrophotometer
• Fluorescence Spectrophotometer
• Comparison Microscope (RCM-505T)
• Trinocular Stereo Zoom Microscope (Model- T- 5MP)

Fourier-transform infrared spectroscopy (FTIR) Make: Perkin Elmer
Model: Spectrum Two

Infrared Spectroscopy serves as a powerful tool for analyzing molecular structures, providing valuable insights into their vibrational and rotational modes of motion. This technique plays a pivotal role in the identification and characterization of functional groups within organic compounds. By generating unique fingerprint spectra, Infrared spectroscopy enables the differentiation of compounds based on their absorption patterns.

The recorded Infrared spectrum serves as a comprehensive record, offering crucial information about both organic and inorganic compound structures. It facilitates the identification of functional groups, and chemical bonding, and aids in the quality control of raw materials. The absorption of specific wavelengths of the IR beam by a sample directly correlates to the presence of particular functional groups within the compound.

Fourier Transform Infrared (FTIR) Spectroscopy has emerged as a sensitive and rapid technique for characterizing materials across a wide range of samples and molecular species. Its high signal-to-noise ratio enhances its utility, especially for challenging sample analyses. FTIR capitalizes on the unique absorption or transmission spectral fingerprints generated by the functional groups of each molecule, enabling precise compound identification.

Increasingly, FTIR is becoming the preferred technology for compound testing due to its speed, accuracy, and reliability. Its ability to swiftly and accurately characterize compounds makes it indispensable for various applications, ranging from material characterization to quality control processes. FTIR's versatility and efficiency make it an invaluable asset in research, development, and industrial settings alike.

Spectrum Two FT-IR spectrometer

The Spectrum Two FT-IR spectrometer is designed to revolutionize your material analysis, offering unmatched ease of use, power, and portability. This compact and robust system delivers exceptional performance in both laboratory and remote settings, making it the ideal choice for a wide range of applications. Spectrum Two simplifies your workflow with its intuitive interface and fully integrated universal sampling.

Key Benefits:

Simple operation: Intuitive interface and automated features streamline analysis. Unmatched versatility: Perform a wide range of applications with ease.

Applications

• Chemical Identification: Analyse paints, adhesives, resins, polymers, coatings, and drugs to determine their composition.
• Contamination Control: Isolate and characterize organic contaminants in materials,
• Material Characterization: Identify mixtures of organic and inorganic compounds, solid or liquid.
• Polymer Analysis: Decipher the building blocks of polymers and their blends
• Surface Trace Detection: Indirectly verify the presence of trace organic contaminants on surfaces,
• Routine Analysis: Perform qualitative and quantitative analyses with ease, s
• Thin Film Studies: Investigate the composition and structure of thin films.

Detector:

• Standard, high-performance, room temperature LiTa3 (Lithium Tantalate) MIR Detector

Window Options:

1. KBr windows:

• Enable data collection over a spectral range of 8300 to 350 cm-1
• Best resolution: 0.5 cm-1
• Ideal for most applications

2. ZnSe windows (for exceptionally humid environments):

• Enable data collection over a spectral range of 6000 to 550 cm-1
• Best resolution: 0.5 cm-1
• Recommended for use in high humidity environments to prevent moisture damage to the detector

Key Points:

• The LiTa3 detector offers a good balance of performance and affordability.
• KBr windows provide a wider spectral range for most applications.
• ZnSe windows are ideal for humid environments to protect the detector from moisture damage, but offer a slightly smaller spectral range.

Sample Requirements

Solid Samples:

• Form: Powder
• Minimum Amount: 1-2 mg

Liquid Samples (Oily Only):

• Form: Oil
• Minimum Amount: 0.1 mL

Thin Films:

• Accepted directly, following specific guidelines provided upon analysis request. Additional Tips:
• For best results, ensure your solid samples are homogenous and finely ground (particle size less than 10 microns).
• If your sample is larger than the recommended amount, a smaller portion will be used for analysis.
• Consult the instrument manual or your service provider

UV/Vis Spectrophotometer

The UV-VIS spectrum is due to the electronic transitions of the molecule. This is characteristic of a compound. Qualitative and quantitative estimations of compounds are possible by this nondestructive technique. The optical absorption by samples in the ultraviolet and visible are measured with this instrument. This region finds wide acceptance in the food and grain industry for the determination of protein, fat, moisture, sugar, etc. Absorption measurements in the UV and visible regions provide information about electronic transitions in a sample For fast and reliable results time after time, choose the LAMBDA 25for routine UV/Vis applications, pharmacopeia and regulatory tests, and liquids analysis. The comprehensive range of operating modes provides trustworthy results for a range of analyses including quantitative measurements and kinetics studies.

Lambda 25 UV/Vis Spectrophotometer

The Lambda 25 UV/Vis Spectrophotometer is designed to streamline your routine ultraviolet-visible (UV/Vis) analyses, delivering dependable results with exceptional ease of use. Caters to a broad spectrum of applications, making it a valuable asset for any laboratory seeking efficient and reliable performance.

The Lambda 25 tackles a wide range of UV/Vis applications, including:

• Quantitative analysis: Accurately determine the concentration of various analytes in solution.
• Qualitative analysis: Identify unknown compounds based on their unique UV/ Vis absorption profiles.
• Kinetic studies: Monitor chemical reactions in real-time by observing changes in absorbance over time.

Key specifications:

• Spectral Range:
• 190 nm (near-ultraviolet) to 1100 nm (visible and near-infrared)
• This broad range allows you to analyze samples that absorb light across a wide spectrum of wavelengths.

Resolution:

• 1 nm
• This exceptional resolution enables you to differentiate between closely spaced absorption peaks, providing detailed information about your sample's composition.
• Bandwidth: 1 nm (fixed)
• Modes Of Operation: scanning, wavelength program, time-drive, rate, quant, scanning quant
• True double-beam operation, High throughput,

Applications:

• Widely used in analytical chemistry for:
• Quantitative analysis: Determining concentrations of various analytes, like transition metal ions, conjugated organic compounds, and biological macromolecules.
• Qualitative analysis: Identifying unknown compounds based on their unique UV/Vis absorption patterns.
• Material studies: Investigating properties of materials, including molecular structure, reaction kinetics, defects in solids, and color centers.
• Samples: Primarily analyzes solutions, but can also be used for solids and gases.

Underlying Principle: Electronic Transitions

• Process: UV or visible light is absorbed by a molecule, promoting an electron from its ground state to a higher energy state.
• Information obtained:
• Conjugation: The number of conjugated double bonds and aromatic character can be determined by analyzing the UV-Vis spectrum.
• Conjugation vs. Non-conjugation: The presence or absence of conjugation can be distinguished based on the absorption pattern.

Fluorescence Spectroscopy

Molecules have various states referred to as energy levels. Fluorescence spectroscopy isprimarily concerned with electronic and vibrational states. Generally, the species being examined has a ground electronic state (a low-energy state) of interest and an excited electronic state of higher energy. Within each of these electronic states are various vibrational states. In fluorescence spectroscopy, the species is first excited, by absorbing a photon, from its ground electronic state to one of the various vibrational states in the excited electronic state. Collisions with other molecules cause the excited molecule to lose vibrational energy until it reaches the lowest vibrational state of the excited electronic state. This process is often visualized with a Jablonski diagram.

Perkin Elmer LS 55 Spectrofluorometer:

A powerful technique for analyzing a wide range of samples. This versatile instrument caters to the demanding needs of researchers in biophysics, chemistry, materials science, and beyond.

Applications, including:

• Measure fluorescence emission and excitation spectra to understand the photophysical properties of your samples.
• Quantify biomolecules like proteins and DNA with high sensitivity.
• Characterize complex materials by investigating their fluorescence properties.
• Study fluorescence lifetimes to gain deeper insights into molecular dynamics.

Specifications:

• Wavelength accuracy + 1 nm
• Wavelength reproducibility Spectral bandpass + 0.5 nm the excitation slits 2.5- 15 nm and emission slits 2.5-20 nm
• Scanning speed,the scanning speed can be selected in increments of 1 nm from 10–1500 nm/minute.
• Data can also be collected with respect to time.
• Emission cut-off filters, 290, 350, 390, 430 and 515 nm,
• Sensitivity Minimum signal-to-noise level using the Raman band of water, excitation 350 nm, is 750:1 RMS measuring noise on the Raman peak, and 2500:1 RMS measuring noise on the baseline

Comparison Microscope (RCM-505T)

The comparison microscope is a specialized tool used in forensic science and investigation for side-by-side comparison of two microscopic specimens. It consists of two compound microscopes linked by an optical bridge that allows simultaneous viewing of two specimens. This setup enables forensic scientists to compare samples, such as fibers, hairs, bullets, tool marks, and documents, to determine if they share common characteristics or if they are identical. comparison microscopes play a crucial role in forensic science and investigation by enabling detailed analysis and comparison of microscopic evidence. Their ability to provide precise and objective comparisons helps forensic examiners in identifying and linking evidence to suspects, victims, or crime scenes, contributing to the investigation and resolution of criminal cases.

Components of a Comparison Microscope:

• Binocular Microscope Heads: Each side of the microscope has a binocular eyepiece for comfortable viewing.
• Objective Lenses: These lenses provide magnification of the specimens. Different objective lenses with varying magnifications can be used based on the requirements of the examination.
• Stage: The stage holds the specimens in place for examination.
• Light Source: A light source illuminates the specimens, typically from below, to enhance visibility.
• Optical Bridge: This component connects the two microscopes, allowing the simultaneous viewing of the specimens.

Benefits:

• Simultaneous Viewing: The ability to view two specimens simultaneously allows for quick and accurate comparisons.
• Enhanced Precision: The high magnification and clarity provided by comparison microscopes enable forensic scientists to identify subtle similarities and differences between specimens.
• Objective Analysis: Comparison microscopy provides an objective method for evaluating evidence, reducing the risk of subjective interpretation.
• Versatility: Comparison microscopes can be used for a wide range of forensic examinations, making them a versatile tool in forensic laboratories.

Application:

• Firearm Examination: Comparison microscopes are extensively used in firearm examination in ballistics to analyze bullets and cartridge cases. Forensic examiners compare striation marks left on bullets and cartridge cases by the barrel of a gun to determine if they originate from the same firearm.
• Tool Mark Analysis: When tools are used in crimes such as burglary or vandalism, they often leave distinctive marks on surfaces. Comparison microscopes help forensic experts compare these tool marks to tools recovered from suspects or crime scenes, aiding in identifying the tool used.
• Fiber and Hair Analysis: Fibers and hairs can be transferred during criminal activities and can provide valuable evidence. Comparison microscopes allow forensic analysts to compare the characteristics of fibers and hairs found at a crime scene with those found on suspects or victims.
• Document Examination: In cases involving forged documents or altered writings, comparison microscopes aid forensic document examiners in comparing the handwriting, ink, paper, and other features of questioned documents with known samples.
• Trace Evidence Examination: Various types of trace evidence, such as paint chips, glass fragments, and soil particles, can be compared using a comparison microscope to link them to a particular source or crime scene.

Trinocular Stereo Zoom Microscope (Model- T- 5MP)

The trinocular stereo zoom microscope is an advanced variation of the stereo zoom microscope, featuring an additional optical port (trinocular head) that allows for the attachment of a camera or other imaging device. This configuration enables users to simultaneously view specimens through the binocular eyepieces while also capturing images or videos of the specimens for documentation, analysis, and presentation purposes. Trinocular stereo zoom microscopes find extensive use in various fields, including forensic science and investigation, due to their versatility and enhanced imaging capabilities. trinocular stereo zoom microscopes are indispensable tools in forensic science and investigation, offering advanced imaging capabilities for documentation, analysis, and comparison of forensic evidence. Their ability to simultaneously view specimens and capture high-resolution images or videos makes them valuable instruments in forensic laboratories and crime scene investigations, contributing to the identification, analysis, and interpretation of forensic evidence.

Components of a Trinocular Stereo Zoom Microscope:

• Binocular Eyepieces: Trinocular stereo zoom microscopes typically feature two binocular eyepieces for comfortable viewing with both eyes, providing a three-dimensional perception of the specimen.
• Zoom Objective Lenses: These lenses can be adjusted to vary the magnification level continuously, allowing users to zoom in and out on the specimen to examine details at different levels of magnification.
• Trinocular Head: The trinocular head contains an additional optical port, allowing for the attachment of a camera or imaging device. It typically has a beam splitter mechanism that directs a portion of the light to the camera while maintaining simultaneous viewing through the eyepieces.
• Illumination: Trinocular stereo zoom microscopes may have built-in illumination sources, such as LED lights, positioned above or below the stage to illuminate the specimen. Adjustable lighting options enhance visibility and contrast for imaging.
• Articulating Arm: These mounting systems provide flexibility in positioning the microscope and allow for easy manipulation of the specimen during examination and imaging.

Benefits Trinocular Stereo Zoom Microscopes:

• Simultaneous Viewing and Imaging: The trinocular configuration enables users to view specimens through the eyepieces while simultaneously capturing images or videos using a camera, enhancing workflow efficiency.
• High-Resolution Imaging: Trinocular stereo zoom microscopes produce highresolution images suitable for detailed documentation and analysis of forensic evidence.
• Versatility: These microscopes can accommodate various imaging devices, including digital cameras, CCD cameras, and digital video cameras, allowing for flexible imaging options based on the specific requirements of forensic examinations.
• Non-Destructive Examination: Trinocular stereo zoom microscopes enable non-destructive examination of forensic evidence, preserving samples while obtaining valuable imaging data.

Applications in Forensic Science:

• Documentation of Evidence: Trinocular stereo zoom microscopes are used in forensic science to document evidence, such as fingerprints, hair, fibers, and tool marks, with high-resolution images or videos. Capturing detailed images of forensic evidence aids in documentation, analysis, and presentation in court proceedings.
• Comparison and Analysis: Forensic investigators use trinocular stereo zoom microscopes to examine and compare evidence, such as ballistic materials, tool marks, and trace evidence. High-quality imaging capabilities allow for detailed analysis of microscopic features and facilitate comparisons between questioned and known samples.
• Fingerprint Examination: Trinocular stereo zoom microscopes are essential for analyzing fingerprints, both latent and visible. Forensic experts can capture clear images of fingerprint impressions, ridge patterns, and minutiae details for identification and comparison purposes.
• Trace Evidence Examination: In cases involving trace evidence, such as soil, glass fragments, or paint chips, trinocular stereo zoom microscopes enable forensic scientists to document and analyze microscopic features. Precise imaging helps in linking trace materials to specific sources or locations.
• Forensic Document Examination: Trinocular stereo zoom microscopes are used by forensic document examiners to examine documents for alterations, forgeries, or counterfeit features. Detailed imaging allows for the analysis of ink characteristics, paper fibers, and printing patterns

HOW TO SUBMIT THE SAMPLES

The samples should be submitted in the prescribed format indicating the information called for as well as your Special requirement if any. If you are sending them by mail, please enclose the payment along with the samples and address the same to:

The Coordinator

PC Ray Research Centre

ITM University, AH 43, Jhansi Road, Turari, Gwalior

Term & Condition:

• Submit samples in air-tight vials.
• Samples should be submitted along with a duly filled requirement form
• Submit samples and work orders separately for each analysis technique
• Users (for educational purposes) should mention the name of the student who needs the analysis.
• e-mail ID of the user is mandatory (SPECIFY THE EMAIL ID LEGIBLY)
• Mention the details of the samples (ingredients, presence of halogen, nitrates, amino group, hydrazine, solvents used for preparation,etc.)

(SAMPLES SUBMITTED WITHOUT THESE DETAILS WILL BE REJECTED)

Only on receipt of the payment along with the samples, they will be registered for analysis and taken upfor measurement as per the seniority/queue of the users of the instrument concerned. As soon as the analysis is over, the spectra along with the receipt/bill will be sent to the users. While submitting samples for more than one analysis clearly specify and label properly in the sample tube.

Important notice:

• The data collected at PC Ray Research Center will find proper attribution (facility, instrument location, and concernedscientists willbe explicitly mentioned) when the same is posted in the public domain in any form
• (Figures, tables numbers quoted in text or characterization meeting standards). This applies to thesis, report, conference presentation & proceeding reference journal papers, etc.
• PC Ray Research Center. ITM University as an institute will be acknowledged for extending its facilities:
• Acknowledgment by designation would be avoided. The concerned scientist will be informed before the Submission for publication to avoid ethical problems. Co-authorship from CSR will be discussed mutually.
• soft copies of all such publication material and details of any thesis / dissertation with such Data will be sentto the concerned PC Ray research center

Mode of Payment:

Please make sure that the payment is received along with the samples. Payments are to be made By crossed-demand draft (DD) or cash payment to the account office. The DD must be drawn in favor of: “The Registrar, ITM University, Gwalior” payable at Gwalior and must be sent along with samples and the Registration form to The Head of PC ray research center, ITM University, Turari, Gwalior

CHARGES FOR EXTERNAL SAMPLE ANALYSIS

The following charges are recommended for using UV Vis spectrophotometer, FTIR, andfluorescence spectrometer (Users from other organizations) in PC Ray Center for Research.

S.No	Name of Instrument	For Educational Institutes	For Industries
1	UV Vis Spectrophotometer	50/Sample	100/Sample
2	FTIR	100/ Sample	150 Sample
3	Fluorescent Spectrophotometer	125 Sample	125 Sample